Environmental, Health and Safety Services

Special Labs

These are laboratories that collect and/or perform minor manipulations with human/NHP blood, tissues or other potentially infectious materials for research or teaching purposes. The scope of activities of such labs may require 1) registration with the IBC and 2) meeting EHS biosafety requirements below, but may not require full protocol submission and review with the IBC. See table below for details.

LAB TYPE MATERIAL AND MANIPULATION (Manipulation = removing material from closed container & doing something with it) REQUIREMENTS for the IBC REQUIREMENTS for BIOSAFETY
Research Lab Blood draw only – no or minor manipulation
  • Contact the IBC.
  • Document training for personnel.
  • Contact IRB.
  • Confirm that lab has contacted the IBC.
  • Lab/area inspection could take place.
  • Complete written procedures + Exposure Control Plan & supply copies in work area.
  • Training: BBP
  • Have in place:
    • EHS phlebotomy guidelines
    • Biohazard door signage
    • Appropriate holding/storage area for labeled specimens
    • Proper lab waste collection/disposal; spill kit
Research Lab Extensive manipulation of human blood, tissues, cell cultures, etc.
  • Register with IBC (full review and approval).
  • Document training for personnel.
  • Contact IRB.
  • Confirm registration with IBC as BSL-2 lab.
  • Biosafety lab inspection will take place.
  • Complete Lab-Specific Biosafety Manual (includes Exposure Control Plan) & supply copy of LSBM in work area.
  • Training: BBP; site-specific training as needed
  • Have in place:
    • BSL-2 door signage
    • Biohazard stickers on equipment
    • Appropriate holding/storage area for labeled specimens
    • Proper lab waste collection/disposal; spill kit
Clinical Diagnostic Labs (e.g., Schiffert, Vet. Med.) Manipulation of tissues/fluids/ cell suspensions that are potentially infective If not involved in generating research data, then lab is not subject to IBC oversight.
  • Follow CDC "Guidelines for Safe Work Practices in Human and Animal Medical Diagnostic Laboratories."
  • If the lab is not accredited or inspected by an outside agency then EHS will inspect it.
Clinical Research Labs (e.g., VTCRI) Urine collection
Saliva collection
Blood collection
  • Contact the IBC.
  • Document training for personnel.
  • Contact IRB.
  • Confirm that lab has contacted the IBC.
  • Lab/area inspection could take place.
  • Complete written procedures + Exposure Control Plan & supply copies in work area.
  • Training: BBP; site-specific training as needed
  • Have in place:
    • Biohazard door signage
    • Appropriate holding/storage area for labeled specimen
    • Proper lab waste collection/disposal; spill kit
Service Labs (e.g., Flow Cytometry, Microscopy) Manipulations of fixed or live tissues, fluids or cell suspensions of:
  • human/NHP material
  • tumorogenic material
  • recombinant material
  • infected or possibly infective material
  • Contact the IBC.
  • Document training for personnel.
  • Confirm that lab has contacted the IBC.
  • Lab/area inspection could take place.
  • Complete written procedures + Exposure Control Plan & supply copies in work area.
  • Training: BBP; site-specific training as needed
  • Have in place:
    • Biohazard door signage
    • Biohazard labels on equipment
    • Proper lab waste collection and disposal; spill kit

DETAIL LIST OF BIOSAFETY REQUIREMENTS FOR SPECIAL LABS:

Signage/labeling:

  • Post Biohazard sign (or Biosafety Level sign) on all access doors.
  • Post any other warnings or requirements to enter, such as 'No eating/drinking'.
  • Place Biohazard labels on 1) equipment used with biohazardous material and 2) storage units (freezers/refrigerators) containing biohazardous material.

Waste Handling:

  • Have in place well-labeled lab waste containers with lids and visible biohazard labels.
  • Use the appropriate bag in the waste container, according to the type of waste being generated.
  • Autoclave biowaste, then dispose of in Regulated Medical Waste.

Disinfection:

  • Ensure that appropriate disinfectant at proper concentration is adequately supplied.
  • Ensure that spill cleanup procedure is posted or readily available, and spill supplies are available.

PPE:

  • Ensure that lab coat and gloves (minimum requirement) are supplied.
  • Based on risk assessment, ensure other necessary PPE is supplied: safety goggles, face shields; respiratory protection may be required to protect against inhalation of aerosols.

Universal Precautions:

  • Ensure that Exposure Control Plan or Lab-Specific Biosafety Manual (inclusive of ECP) is in place.

Training:

  • Online EHS Bloodborne Pathogens training for lab workers: See 'Training' section.
  • Site-specific training is dependent on the equipment, work procedures, material and hazards involved; Principal Investigators, lab managers, or area supervisors or their designees are responsible for delivering site-specific training.
  • Records – Documentation of all training must be kept current, and maintained for 3 years post-employment.

Documentation:

  • Exposure Control Plan – complete, and make a copy available for reference and training.
  • Written procedures involving biohazardous material – complete and include containment practices and safe handling/sharps/storage/disposal measures; make copies available for work area.

Further Information and Guidance:

IBC
EHS Biosafety Group